Assessment of ovarian tissues autografted to various body sites followed by IVM in mouse

Ovarian tissue transplantation is emerging technologies for fertility preservation. In addition, in vitro maturation [IVM] of oocytes retrieved from ovarian tissues may overcome the fertility defects in certain cases. The aim was to evaluate the best site for ovarian tissue transplantation in mice. Also, feasibility of IVM of oocytes retrieved from auto grafted ovarian tissues was freshly assessed. Hemi-ovaries from 6 weeks old mice were auto grafted into kidney capsule [K] versus the back muscle [B] and leg muscle [L] in a mouse auto graft model which was stimulated with gonadotrophins. Then ovarian grafts were recovered and processed histologically for follicle assessment compared with control, also the ability of oocytes to mature with IVM was studied 14 days after transplantation. Total follicle count was significantly higher in K-graft [3.5 +/- 3.17] and the antral follicles were only observed in K-site model. The number of retrieved immature oocytes as well as successful IVM in K-grafts was significantly higher than other groups [p=0.008, p=0.016]. The kidney capsule is a promising site for ovarian tissue auto graft in mice. This resulted in better follicular survival and IVM outcomes

[ view points of medical students in yazd university of medical sciences toward horizontal integration teaching method in anatomical sciences courses]

Background and Objective: At present two teaching methods of traditional and integration are used for the theoretical instruction of anatomical sciences including anatomy, histology and embryology. It is thought that the integration teaching method can help Medical students, especially in their clinical course of study. This study was designed to find the attitudes of Medical students of Yazd University of Medical Sciences toward this method in teaching anatomical sciences courses

Methods: This study was done on Medical students who had been educated by the integration method. Data collection was done by using a self administrated questionnaire in four domains of knowledge about the integration program of anatomical sciences courses, facilities of the integration process, order of course presentation and satisfaction of the integration plan. Data were analyzed through SPSS software package

Results: Most of the respondents had moderate familiarity with the integration method and asserted that this method had moderately facilitated their learning process in anatomical sciences courses. According to most participants, the rate of coordination between the organ system was moderate. Students' satisfaction of integration of embryology, histology and anatomy courses was high. In regard to the order of course presentation, participants preferred histology, embryology, anatomy, and physiology order

Conclusion: Attitudes of Medical students toward horizontal integration method of basic sciences courses was moderate and based on the obtained results, it seems that the best presentation order is respectively histology, embryology, anatomy, and physiology?